LS - An International Journal of Life Sciences
  • Year: 2014
  • Volume: 3
  • Issue: 3

16S rRNA Gene-based Identification and Antibiotic Resistance Profiling of Acinetobacter baumannii from River Water Isolate, Dehradun, India

  • Author:
  • Sadar Ali1, Rakesh Kumar Gupta2, Heena Shoket3, Vinod Kumar Mishra4,
  • Total Page Count: 6
  • Published Online: Dec 1, 2014
  • Page Number: 157 to 162

1Department of Biotechnology, Doon P.G. College of Agriculture Science & Technology, Dehradun-248001, Uttarakhand, India

2Department of Biotechnology, Doon P.G. College of Agriculture Science & Technology, Dehradun-248001, Uttarakhand, India

3Department of Biotechnology, Doon P.G. College of Agriculture Science & Technology, Dehradun-248001, Uttarakhand, India

4Department of Biotechnology, Doon P.G. Paramedical College, Dehradun-248001, Uttarakhand, India

*Corresponding author e-mail id: mishravkbhu@gmail.com

Abstract

Acinetobacter baumannii represents the most clinically relevant opportunistic pathogen. This study describes to 16S rDNA sequence-based identification of Acinetobacter spp. from river water. The strain showed 99% identity with that of several A. baumannii reference strains in GenBank. The phylogenetic analysis indicated that A. baumannii W29 strain was related to strains within the A. baumannii, and yet have a distinct line of descent, representing a novel species. The study revealed multi drug resistance in the species to cephalosporins, carbapenems and other classes of antibiotics (Pencillins,p-Lactam/3-Lactamase inhibitor combinations, aminoglycosides). The occurrence of A. baumannii in river water sources has serious epidemiological implications and a potential human health risk.

Keywords

Acinetobacter baumannii, Antibiotic resistance, Microbes, PCR, Phylogeny, 16S rRNA, Water