1Assistant Professor, Plant Bioenergetics and Biotechnology Laboratory, Department of Botany, Mohanlal Sukhadia University, Udaipur, Rajasthan, India
2M.Sc. Student, School of Life Science, Jaipur National University, Jaipur, Rajasthan, India
3Former Professor, Department of Botany, University of Rajasthan, Jaipur, Rajasthan, India
*Corresponding author email id: vineetsonijnu@gmail.com
The effects of polyethylene glycol (PEG)-induced drought stress were examined on the activities of various key enzymes that is peroxidase, nitrate reductase, acid phosphomonoesterase, invertase in Cyamopsis tetragonoloba of variety RGC-197. Drought-stress-induced decrease in the activities of nitrate reductase, peroxidase, α-amylase and invertase was observed in leaves of Commiphora wightii. The increased activity of peroxidase enzyme in plants under in vitro water stress condition indicates that the plant is capable of maintaining growth vigour despite adverse conditions. On the other hand, acid phosphomonoesterase activity increased in seedlings germinated on MS medium containing 2% and 3% of PEG. Biochemical analysis indicates that RGC-197 variety of cluster bean is quite resistant and well-developed to endure drought stress by modulating the biosynthesis of proline and expression of various key enzymes, such as enzymes peroxidase, nitrate reductase, invertase and acid phosphomonoesterase. The study will be highly beneficial in development of transgenic plants against drought stress.
Acid phosphomonoesterase, Cyamopsis tetragonoloba, In vitro drought, Invertase, Nitrate reductase, Peroxidase, Polyethylene glycol
