LS - An International Journal of Life Sciences
  • Year: 2018
  • Volume: 7
  • Issue: 3

Identification of Constitutive Amino Acid Residues in the Active Site Domain of Polygalacturonase of Mucor circinelloides ITCC 6025

1Ph.D. Student, Department of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla-171005, Himachal Pradesh, India

2Professor, Department of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla-171005, Himachal Pradesh, India

(*Corresponding author) email id: *reenagupta_2001@yahoo.com

**me2akhilesh@gmail.com

Abstract

Polymerase chain reaction-amplified polygalacturonase (PG) gene from Mucor circinelloides ITCC 6025 was sequenced partially and nucleotide sequence of 490 bp was obtained. It was translated to the corresponding amino acid sequence by using Swiss-Prot program. Blastp algorithm revealed a maximum identity of 99% with homologous sequences deposited in the data bank of National Centre for Biotechnology Information. The amino acid sequence alignment of PGs by ClustalW2 programme exhibited strictly conserved aspartic acid (Asp178), histidine (His229), arginine (Arg262) and lysine (Lys264) residues.

Keywords

Amino acids, Blastp, ClustalW2, Polygalacturonase, Sequencing, Swiss-Prot, Mucor