Comparative Studies of DNA and Protein of Staphylococcus aureus Growing in Different Environmental Conditions

Environment is known to influence organisms. The impact of environmental factors on growth, morphology, reproduction etc. has been reported for several organisms but scant attention has been given to impact on genome and protein expression. In the present study Staphylococcus aureus growing in different environmental conditions were studied for variation in DNA and protein. The two different Staphylococcus aureus were isolated from agricultural and saltpan soils. Biochemical tests were performed for confirming the presence of Staphylococcus aureus. After confirming, genomic DNA was isolated. RFLP analysis and SDS-PAGE were also done to compare the single organism growing in different environmental conditions. Although S. aureus growing in different environmental conditions shows similar characteristics at morphological, biochemical and DNA molecular weight level, there was a difference observed in the protein expression level.