Medico-Legal Update

  • Year: 2018
  • Volume: 18
  • Issue: 2

Comparison of two DNA extraction Methods and their Utility in Forensic examination of Bone samples

1Senior Scientific Officer, Biology Division Forensic Science Laboratory Home Department, GNCT of Delhi, Rohini, Delhi, India

2Assistant Professor, Amity Institute of Forensic Science, Amity University, Noida, India

3Assistant Director, Biology Division Forensic Science Laboratory Home Department, GNCT of Delhi, Rohini, Delhi, India

4Professor and Head, Amity Institute of Forensic Science, Amity University, Noida, India

Online published on 21 July, 2018.

Abstract

The bones frequently present as an important source of DNA available for the identification of mutilated human remains especially in the mass disaster situations. The calcareous nature of bones resists the decomposition making them a preferred source of DNA. This paper deals with he comparative analysis of quality and quantity of DNA extracted by magnetic beads based Prepfiler BTA extraction method and manual organic extraction methods and their utility in forensic examinations of bone samples. The study was carried out on 20 samples and it was found that the quantity of DNA isolated by automated extraction method was in the range of 0.7 to 4.45ng ng/μl and the success rate was 90% as compared to the quantity of DNA isolated through organic extraction method was in the range of 0.01 to 1.36 ng/μl and success rate was 35%, hence proving that the magnetic bead based automated method of DNA extraction is quite useful in forensic examination.

Keywords

DNA extraction, organic methods, magnetic beads, bones, human identification etc