Phytopathogenic mollicutes
SCOPUS
  • Year: 2025
  • Volume: 15
  • Issue: 1

Characterization of the membrane protein of onion yellows phytoplasma that has a domain of Deg/HtrA family protease

  • Author:
  • Hidemasa Oda1, Endo Ai1, Takahiro Sambe1, Kensaku Maejima2, Shigetou Namba2, Yasuyuki Yamaji2, Kenro Oshima1,*
  • Total Page Count: 2
  • Published Online: Mar 5, 2025
  • Page Number: 3 to 4

1Department of Clinical Plant Science, Faculty of Bioscience and Applied Chemistry, Hosei University, Tokyo, Japan

2Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan

*Corresponding author e-mail: Kenro Oshima (kenro@hosei.ac.jp)

Online published on 5 March, 2025.

Abstract

Since phytoplasma resides intracellularly within a host plant cell and lacks a cell wall, its membrane proteins may have important roles in the host-phytoplasma interaction. DegQ-like protein of onion yellows phytoplasma (OY-DegQ) is a membrane protein that contains a Deg/HtrA family serine protease domain and shows partial similarity to Escherichia coli DegQ. To examine the protease activity of OY-DegQ, the soluble domain of OY-DegQ was purified with glutathione S-trans- ferase and its function was analyzed. As a result, the degradation of a substrate protein was observed, suggesting that OY-DegQ functions as a protease.

Keywords

Phytoplasma, Serine protease, Enzymatic activity, Plant disease