Phytopathogenic mollicutes

“Flavescence dorée” (FD) is an epidemic quarantine disease of grapevine in Europe efficiently spread by the leafhopper Scaphoideus titanus. Promptly uprooting infected stocks and insect control are mandatory. Systematic monitoring of FD has also to be conducted in FD-free areas. However, in these regions the non-quarantine disease “bois noir” (BN), which induces similar symptoms in grapevine as FD, is widespread. Therefore, fast and reliable detection methods for FD monitoring in the field had to be developed. The concept of the PhenoTruck® is based on three axes: large-scale screening of vineyards using remote sensing by drones (UAVs), hyperspectral screening of leaf samples for phytoplasma presence and molecular identification of FD phytoplasmas in a mobile laboratory. The latter is a special vehicle with 4-wheel drive which allows autonomous laboratory work direct at the field. Drone image data are automatically processed and sample strategies developed. One compartment of the mobile laboratory is equipped with a dual hyperspectral camera system (VNIR+SWIR, wavelength range from 400 - 2,500 nm). The spectra of leaf samples are automatically analyzed for phytoplasma presence. Using machine learning technologies, phytoplasma-infected and healthy leaves as well as phytoplasma symptoms and other biotic and abiotic stress factors could be distinguished. In addition, the spectral discrimination of FD- and BN-infected leaves was achieved. The molecular identification of FD-infections was done by improved LAMP assays. The concept of the mobile laboratory PhenoTruck® is open for any other plant disease detection.