Recombinase polymerase amplification assay: A simplified and rapid approach for detection of fastidious plant pathogens

Plant pathogens threaten global food security, by causing substantial yield losses in several economically important crops. The early detection and identification of pathogens is of utmost importance to control the spread of such plant pathogens and the emergence of new diseases, reducing the associated agricultural losses. Traditional detection methods are time-consuming and require specialized equipment. Recombinase Polymerase Amplification (RPA) is an isothermal amplification technique which offers a rapid and sensitive detection of plant pathogens directly from plant samples. By eliminating the need for thermal cycling and DNA extraction, RPA simplifies the detection process and enables on-site pathogen detection. The present study highlights the potential of RPA as a powerful tool for early detection and effective disease management of fastidious pathogens infecting commercially important crops.