Medicinal Plants - International Journal of Phytomedicines and Related Industries

A total 30 accession of Cleome viscosa L. were conserved at Indian National Genebank, ICAR-NBPGR. Poor seed germination was observed at the time of testing for viability during processing of germplasm for long-term conservation. To improve germination, seeds collected from different sources (provenances) were treated with various concentration of H₂SO₄, scarification, hot water treatment and co-application of GA₃. Treatments with 100% H₂SO₄ for 20 min. significantly enhanced (P <0.001) percent seed germination, irrespective of provenance. Co-application of GA₃ was found ineffective in improving per cent seed germination.