1Department of Medicinal and Aromatic Crops, Horticultural College and Research Institute, Tamil Nadu Agricultural University, Coimbatore-641003, Tamil Nadu, India
2Department of Medicinal and Aromatic Crops, Horticultural College and Research Institute, Tamil Nadu Agricultural University, Coimbatore-641003, Tamil Nadu, India
3Department of Spices and Plantation Crops, Horticultural College and Research Institute, Tamil Nadu Agricultural University, Coimbatore-641003, Tamil Nadu, India
4Department of Plant Genetic Resources, Tamil Nadu Agricultural University, Coimbatore, 641003, Tamil Nadu, India
5Department of Biochemistry, Tamil Nadu Agricultural University, Coimbatore, 641003, Tamil Nadu, India
*Corresponding author e-mail: bharathiudhaya.m@gmail.com; med@tnau.ac.in
Online published on 4 October, 2019.
In 25 genotypes of vetiver genotypes, 15 Inter Simple Sequence Repeats (ISSR) primers were examined for genetic diversity analysis. A total of15 ISSR markers were screened in which 11 showed amplification. Similarity between the genotypes ranged from 0.17–0.90. Mean of percentage polymorphism and polymorphic information content (PIC) was 79.4% and 0.36, respectively. Unweighted Pair Group Method of Arithmetic Mean (UPGMA) derived dendrogram was constructed from the ISSR marker study and discriminated the genotypes into different clusters. Our results suggested that DNA based markers of ISSR could be effectively used for genetic diversity evaluation in vetiver germplasm and for vetiver improvement breeding programme.
Chrysopogon zizanioides, genetic diversity, ISSR, PIC, vetiver
