1Traditional Medicine and Materia Medica Research Center, Shahid Beheshti University of Medical Sciences
2Traditional Medicine and Materia Medica Research Center, Shahid Beheshti University of Medical Sciences, 1516745811, Tehran, Iran
3Department of Traditional Pharmacy, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, 1516745811, Tehran, Iran
*Corresponding author e-mail: mhmoghadam@sbmu.ac.ir
Online published on 23 January, 2020.
Plants from Asteraceae family have been reported with cytotoxic and apoptotic properties. In the present study, Achillea filipendulina, a member of this family was selected for assessment of its toxic potential on cancerous cell lines. Methanol extract of A. filipendulina was evaluated for cytotoxicity by MTT assay in MCF-7 and MDA-MB-468 breast cancer cell lines. The ability of the extract to induce programmed cell death was examined with annexin V assay. The alterations in SubG1 fraction in the breast cancer cell cycle were also evaluated. A. filipendulina demonstrated cytotoxicity on both cell lines with IC50 values of 386 and 248 μg/mL against MCF-7 and MDA-MB-468, respectively. The early and late apoptotic cells were detected in annexin V assay and; increase in subG1 was observed in the cell cycle analysis for both breast cancer cell lines. Our results revealed that A. filipendulina is capable of inducing cell death via apoptosis.
Achillea filipendulina, annexin V, apoptosis, flow cytometery, MCF-7, MDA-MB-468, MTT assay
