Evaluation of in vitro and in vivo anti-inflammatory activity of flavonoid extract of Thespesia populnea and Tabernaemontana divaricata leaves in Swiss albino mice

In the present study, the flavonoid fractions of Thespesia populnea and Tabernaemontana divaricata leaves were evaluated for anti-inflammatory potential. The maximum amount of flavonoids was found in ethyl acetate extract of T. populnea and T. divaricata. The in vitro and in vivo anti-inflammatory activity of the flavonoid fractions were determined by using the membrane stability method and Carrageenan induced oedema (acute), Formalin and Dextran induced oedema (chronic) method respectively. Diclofenac was used as a standard drug. The results revealed that the flavonoid fractions of the selected plants showed the highest membrane stabilizing activity compared to that of standard diclofenac drugs. The flavonoid fractions of both the plant exhibited a significant in vitro and in vivo anti-inflammatory activity.