Evaluation of cinnamaldehyde and cinnamon aqueous extract effects on oxidative stress biomarkers and antioxidant enzymes activity in 5637 bladder cancer cells

The growing trend of bladder cancer around the world has become a major concern. Treatment based on herbal compounds has always been considered for its low side effects and high usefulness. In this study, the effect of cinnamaldehyde and cinnamon aqueous extract on oxidative stress biomarkers and antioxidant enzymes activity in bladder cancer carcinoma, 5637 cell line, was investigated. 5637cell lines, were treated with different concentrations of cinnamaldehyde and cinnamon aqueous extract. MTT was used to evaluate cell viability at 24 h. The concentrations of 0.02, 0.04 and 0.08 mg/ml for cinnamaldehyde and 1.25, 2.50 and 5mg/ml for aqueous extract of cinnamon were selected. Reactive oxygen species (ROS), malondialdehyde (MDA), total oxidant status (TOS), and oxidative stress index (OSI)were significantly higher in cell treated with both cinnamaldehyde and cinnamon aqueous extract than the control group (p<0.05), while in the treated groups total antioxidant capacity (TAC)and uperoxide dismutase (SOD) inhibition rate, glutathione peroxidase (GPx) activity and catalase (CAT) activities were significantly lower than control group (p<0.05). According to the results of the present study, it seems that both cinnamaldehyde and cinnamon aqueous extract could be effective in cancer therapy by increasing of ROS and reduction of antioxidant enzymes activity in bladder cancer.