Estimation of secondary metabolites, antioxidant potential and RP-HPLC analysis of Typha angustifolia

Typha angustifolia is a perennial aquatic weed of the Typhaceae family commonly found in soggy locations and used to treat kidney stones, diarrhoea, tapeworm infection, and abnormal uterine bleeding. This study quantified the aqueous and methanolic leaf extracts of plants for primary and secondary metabolites. The RP-HPLC method was developed for naringin estimation, and antioxidative potential was assessed using DPPH, FRAP, hydrogen peroxide, and phospho-molybdenum free radical scavenging activity. The phytochemical analysis revealed the presence of alkaloids, amino acids, carbohydrates, flavonoids, proteins, tannins, and total polyphenolics to a significant extent. The methanolic leaf extracts showed higher amounts of total phenols and flavonoids. The species in-vitro antioxidant potential, clearly demonstrated that the methanolic extract shows prominent antioxidant properties. The RP-HPLC method revealed the substantial presence of naringin. This study suggested that species may be an effectual antioxidant with significant potential. The RP-HPLC technique designed to quantify naringin was reliable, accurate, repeatable, and specific.