Medicinal Plants - International Journal of Phytomedicines and Related Industries

SCOPUS
  • Year: 2025
  • Volume: 17
  • Issue: 2

Anti-inflammatory potential of Camellia sinensis L. extract in an LPS-induced ARDS lung cell model via modulation of CCL-2, CXCL-9 and IFN-γ expression

  • Author:
  • Rizal Azis1,2,*, Wahyu Widowati3, Didik Priyandoko4, Hanna Sari Widya Kusuma2, Dwi Nur Triharsiwi2, Aris Muhamad Nurjamil2, Vini Ayuni2, Dhanar Septyawan Hadiprasetyo2,5, Marisca Evalina Gondokesumo6, Nguyen Bao Quoc7
  • Total Page Count: 8
  • Published Online: Jul 25, 2025
  • Page Number: 323 to 330

1Biomedical Engineering, Department of Electrical Engineering, Faculty of Engineering, Universitas Indonesia, Depok, 16424, Indonesia

2Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung, 40163, Indonesia

3Faculty of Medicine, Maranatha Christian University, Bandung, 40164, Indonesia

4Biology Study Program, Faculty of Mathematics and Natural Science Education, Universitas Pendidikan Indonesia, Bandung, 40154, Indonesia

5Faculty of Pharmacy, Universitas Jenderal Achmad Yani, Cimahi, 40525, Indonesia

6Faculty of Pharmacy, University of Surabaya, Surabaya, 60293, Indonesia

7Faculty of Biological Sciences, Research Institute of Biotechnology and Environment, Nong Lam University, Ho Chi Minh City, 700000, Vietnam

Abstract

Acute Respiratory Distress Syndrome (ARDS) is a severe and potentially life-threatening condition characterized by alveolar-capillary inflammation, leading to lung injury, pulmonary edema, and respiratory failure. Acute Respiratory Distress Syndrome (ARDS) poses a significant challenge in ICUs due to high incidence and high mortality rates, despite advances in medical care. Effective management and treatment strategies are crucial to improving outcomes for patients with ARDS. This study investigates the anti-inflammatory effects of Camellia sinensis L. extract (CSE), rich in epigallocatechin gallate (EGCG), on inflammation-related gene expression in ARDS lung cell model treated with lipopolysaccharide (LPS). Rat alveolar epithelial type II (L2) lung cells induced by LPS were used as the ARDS model and treated with various concentrations of CSE. The gene expressions of CCL-2, CXCL-9, and IFN-γ were analyzed by qRT-PCR. CSE treatment decreased expression of pro-inflammatory genes CCL-2, CXCL-9, and IFN-γ 1.56 μg/ml CSE concentration (p < 0.05). The results of the study revealed that CSE exhibited potential as an anti-inflammatory agent by inhibiting key inflammatory mediators, suggesting its therapeutic potential in managing ARDS.

Keywords

Cytokines, Inflammation mediators, Phytotherapy, Plant extracts, Polymerase chain reaction