1Department of Food Technology, Faculty of Engineering, JCT College of Engineering and Technology, Pichanur, Coimbatore-641105, Tamil Nadu, India
2Department of Biotechnology, Faculty of Engineering, Karpagam Academy of Higher Education (Deemed to be University), Pollachi Main Road, Eachanari Post, Coimbatore, Tamil Nadu, India
3Department of Microbiology, St. Joseph’s College of Arts and Science (Autonomous), Cuddalore-1, Tamil Nadu, India
4Department of Biotechnology (Bionanotechnology), Centre for Bioscience and Nanoscience Research, Eachanari, Coimbatore-641021, Tamil Nadu, India
*Corresponding author e-mail: satheesh88in@gmail.com
Online published on 25 July, 2025.
Nigella sativa, often termed black cumin, is claimed to have therapeutic benefits namely bactericidal, anti-inflammatory, and antioxidant properties. Hydro distillation was employed to obtain essential oil that emanates from Nigella sativa seeds. The biologically active elements were identified by employing Mass spectroscopy-gas chromatography. The essential oil’s anti-inflammatory performance were assessed by protein denaturation and trypsin inhibition assays, whereas the DPPH assay was used to assess its antioxidant capacity. Furthermore, MTT assay performed to identify the cytotoxic effects on MCF-7 cell lines. The results demonstrated the presence of key bioactive compounds with promising anti-inflammatory and cytotoxic properties. These findings suggest that N. sativa essential oil holds potential as a therapeutic agent with applications in anti-inflammatory and anticancer treatments.
Nigella sativa, GC-MS analysis, Antioxidant, Antimicrobial, MCF-7 cell lines
