Medicinal Plants - International Journal of Phytomedicines and Related Industries

SCOPUS
  • Year: 2010
  • Volume: 2
  • Issue: 2

In vitro evaluation of Actinodaphne hookeri leaf extracts for antioxidant property

1Department of Pharmacognosy & Phytochemistry, K.L.E.S's College of Pharmacy, Vidyanagar, Hubli Karnataka, India.

2Department of Chemistry, Sahyadri Science College, Shimoga, Karnataka, India.

3Department of Industrial Chemistry, Kuvempu University, Jnana Sahyadri, Shimoga, Karnataka, India.

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Abstract

Presence of certain phytoconstituents was studied by preliminary phytochemical investigations and TLC. Various in vitro models were applied to evaluate antioxidant property of petroleum ether, chloroform and alcoholic extracts of Actinodaphne hookeri leaf. In vitro studies include Free Radical Scavenging Capacity (RSC) on DPPH radicals, scavenging capacity on hydroxyl radicals (by measuring the degradation of 2-deoxyribose with OH radicals, generated in Fenton reaction), total antioxidant capacity and scavenging capacity on nitric oxide radicals. Ascorbic acid and Butylated Hydroxy Toluene (BHT) were used as standards. The alcoholic extract showed the maximum free radical scavenging capacity as compared to other extracts and the results were also comparable with that of standard.

Keywords

Free radical scavenging capacity, flavonoids, antioxidants, Actinodaphe hookeri