Medicinal Plants - International Journal of Phytomedicines and Related Industries
SCOPUS
  • Year: 2011
  • Volume: 3
  • Issue: 1

In vitro clonal propagation of Centella asiatica (L.) Urban using shoot tip explants pre-treated with thidiazuron

  • Author:
  • Tanavat Kaensaksiri1, Puangpaka Soontornchainaksaeng2, Noppamas Soonthornchareonnon3, Sompop Prathanturarug1,
  • Total Page Count: 6
  • Page Number: 47 to 52

1Department of Pharmaceutical Botany, Faculty of Pharmacy, Mahidol University, Bangkok, 10400, Thailand

2Department of Plant Science, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand

3Department of Pharmacognosy, Faculty of Pharmacy, Mahidol University, Bangkok, 10400, Thailand

*Corresponding author: Sompop Prathanturarug E-mail: pyspr@mahidol.ac.th

Abstract

A protocol has been established for clonal propagation of Centella asiatica (L.) Urban, a medicinal plant recommended for wound healing. A shoot induction rate of 8.9 shoots/responding explant was obtained when shoot tip explants were cultured in liquid Murashige and Skoog (MS) medium with 4.54 μM thidiazuron (TDZ) for 15 days before being transferred to semisolid MS without plant growth regulator for 10 weeks. Rooting spontaneously occurred. The regenerated plants were successfully transplanted to soil under greenhouse conditions. The regenerants showed uniformity in triterpenoids and ploidy level with their mother plant.

Keywords

Centella asiatica, Apiaceae, micropropagation, ploidy level, thidiazuron