In vitro flowering in Bacopa monnieri (L.) Pennel and the influence of growth regulators, genotype, explant and its physiological age

The present study reports, for the first time, the induction of in vitro flowering in Bacopa monnieri on MS medium supplemented with 3.0 mg/l Kinetin. In vitro flowers were normal in size, shape and violet in color. Among the five growth regulators tested, Kinetin (3.0 mg/l) alone induced in vitro flowering. Out of three explants – leaf, node and internode tested, only node responded positively. When eight accessions, collected for M.P. were compared, only three accessions- BMG-5, BMG-6 and BMG-7 responded positively with maximum response in BMG-6 (20.9%) followed by BMG-7 and BMG-5. All the five nodes of BMG-6 gave positive response with greater percentage from 2^nd node (21.0), while in other two accessions, only 5^th (BMG-5) and 3^rd (BMG-7) node showed in vitro flowering.