1Amity Institute of Microbial Technology, Amity University, Sector-125, Noida, Uttar Pradesh
2Amity Institute for Herbal and Biotech Products Development, Amity University, Sector-125, Noida, Uttar Pradesh
3Amity Institute for Herbal Research and Studies, Amity University, Sector-125, Noida, Uttar Pradesh
1 Corresponding author: Harsha Kharkwal e-mail: hkharkwal@amity.edu
An increase in demand for medicinal herbs and their recognized secondary metabolites as active ingredients has been observed in recent decades. Due to boom in herbal industry an overuse of herbs has lead to extinction or has lowered the supply of lead compounds. Various analytical techniques have been employed to maximize the yield of the desired active compounds. The present research concentrates on enhancement of biomass and secondary metabolite, diosgenin from medicinally important plant Trigonella foenum graecum after interaction with symbiotic fungus, Piriformospora indica. Interaction studies were carried out in green house conditions. Observable fungal root colonization was determined after 7 days of plant root and microbe interaction. A comparison of several growth parameters like shoot and root length; fresh and dry weight; leaf area; chlorophyll contents estimation was assessed after 60th day of plant growth. A significant overall biomass enhancement of the treated plants was observed in comparison with the controls. An increase in secondary metabolites mainly diosgenin was reported by HPTLC analysis.
Piriformospora indica, Trigonella foenum graecum, Biomass enhancement, Secondary metabolites, Root colonization, HPTLC analysis, Transplant shock
