Serological and PCR detection of Garlic common latent virus associated with garlic accessions in India
Abstract
Cloves of twenty one garlic accessions from Indian Agricultural Research Institute, New Delhi and three local garlic lines from Kolar (Karnataka), Anand (Gujarat) and Pantnagar (Uttarakhand) were collected and tested for the presence of Garlic common latent virus (GarCLV). The occurrence of GarCLV was detected by direct antigen coated enzyme linked immunosorbent assay (DAC-ELISA) performed with polyclonal antiserum to GarCLV (Bioreba, Reinach, Switzerland) and immunosorbent electron microscopy (ISEM) after trapping with GarCLV specific antiserum. Reverse Transcription polymerase chain reaction (RT- PCR) using a primer pair 5’ - ATGTCARYGAGTGAAACAGA-3’ and 5’-CTAGTCTGCATTGTTGGATCC-3’ designed from the conserved region of open reading frame 5 (ORF5), which codes for coat protein (CP) of GarCLV, confirmed that all twenty four garlic accessions tested positive for GarCLV. Our study demonstrated wide spread association of GarCLV in garlic accessions including commercial variety, local farmer's cultivars and breeding lines. The potential impact of GarCLV infection on garlic production needs to be addressed.
Keywords
Garlic, Garlic common latent virus (GarCLV), detection, RT-PCR, DAC-ELISA, ISEM, Coat Protein
