Influence of plant growth regulators on the in vitro morphogenetic and callogenetic competence of Adhatoda vasica Nees

This paper describes the effect of plant growth regulators (PGRs) on in vitro responses of nodal explants of Adhatoda vasica. Murashige and Skoog's (MS) medium containing 1.0 mg L⁻¹6-Benzylaminopurine (BAP) and 0.05 mg L⁻¹ α-Naphthalene acetic acid (NAA) showed significantly higher establishment of the nodal explant. Kinetin (KIN) resulted in establishment and proliferation of shootlets. Addition of 0.05 mg L⁻¹NAA and 0.1 mg L⁻¹BAP alone and combination of 0.05 mg L⁻¹NAA + 0.1 mg L⁻¹BAP + 0.1 mg L⁻¹KIN formed shootlets. The axenic shoots in the multiplication and elongation medium fortified with 0.05 mg L⁻¹NAA + 1.0 mg L⁻¹BAP gave multiple shoots and elongation of the shootlets. A combination of 0.05 mg L⁻¹NAA + 1.0 mg L⁻¹KIN showed synergistic effect on elongation of leaves and petioles. In vitro root induction, proliferation and elongation were observed with 0.5 mg L⁻¹Indole-3-butyric acid. Significant callus induction and proliferation ensued with the treatments -0.05 mg L⁻¹NAA + 0.1 mg L⁻¹BAP; 0.05 mg L⁻¹NAA + 0.1 mg L⁻¹BAP + 0.1 mg L⁻¹KIN; and 1 mg L⁻¹ 2,4-(2,4-Dichlorophenoxy acetic acid).