Liquid chromatography– electro spray ionization mass spectroscopic analysis of Acacia species

A rapid liquid chromatography electrospray ionization mass spectrometry (LC-ESI-MS) with positive ion detection method was developed and validated to determine major compounds present in the active fractions of three Acacia species. Different extracts of these three Acacia species were screened for their free radical scavenging activity. The total phenolic content was also determined in all the fractions of three species and identified methanolic fraction as active against free radicals. LC-MS analysis was carried out on methanolic fractions and identified masses of major compounds present.