1Horticultural Biotechnology Laboratory, KNK-College of Horticulture, RVS Agricultural University, Mandsaur-458001, Gwalior, Madhya Pradesh, India
2Department of Medicinal and Aromatic Plants, KNK-College of Horticulture, RVS Agricultural University, Mandsaur-458001, Gwalior, Madhya Pradesh, India
3Department of Plant Pathology, KNK-College of Horticulture, RVS Agricultural University, Mandsaur-458001, Gwalior, Madhya Pradesh, India
4Department of Biochemistry, KNK-College of Horticulture, RVS Agricultural University, Mandsaur-458001, Gwalior, Madhya Pradesh, India
*Corresponding author: Gyanendra Tiwari e-mail: drgyanendratiwari@gmail.com
Online published on 25 March, 2015.
Plumbagin is the major alkaloid responsible for medicinal activities of Plumbago zeylanica. Experiments were conducted to quantify secondary metabolite (plumbagin) production in calli/cell clumps/embryoid acquired from cell suspension culture of P. zeylanica. Higher plumbagin amount was detected in one-month-old calli/cell clumps/embryoid fraction (0.428mg/100gm), liquid culture medium fraction free of calli/cell clumps/embryoid (0.357 mg.L−1) acquired from cell suspension culture as well as in two-months-old rhizogenic calli (1.257 mg per gm) on culture medium MS3D.5IB ((MS+3.0 mgL−1 2,4-D + 0.5 mgL−1 IBA). Linearly increased plumbagin concentration in both fractions of cell suspension culture was recorded with increased concentration of 2,4-D. MS2D.5IB (MS+2.0 mgL−1 2,4-D + 0.5 mgL−1 IBA) was found the best culture medium in increasing growth and rhizogenic calli response in liquid medium.
Plumbago zeylanica, callus, cell suspension culture, secondary metabolites, plumbagin
