1Department of Genetics and Plant Breeding, Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar-385506, Gujarat, India
2CERW, SD Agricultural University, Vijapur, Gujarat, India
3Central Horticultural Experiment Station (CIAH), Vejalpur, Panchmahals, Gujarat, India
4Department of Plant Molecular Biotechnology, Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar-385506, Gujarat, India
*Corresponding author: A.H. Rathod, e-mail: avinashrthd2@gmail.com
Online published on 30 December, 2015.
The mean size of pollen grains of Morinda tomentosa varied in different media with comparatively larger size in distilled water. The pollen viability varied between 83.47 to 95.53% among the accessions. Fifteen to twenty per cent solo sucrose medium appeared precise for pollen germination. The boric acid addition irrespective of concentration (0.005 and 0.01%) in sucrose medium of different concentrations impacted the pollen germination. The germination was highest (85.26%) when 0.005% boric acid was added to 10% sucrose solution. Further, the lower concentration of boric acid (0.005%) evinced better germination of pollen grains than higher concentration of boric acid. The average pollen tube length was maximum (62.42 μ) in 15% sucrose solution while it was minimum (14.36 μ) in 30% sucrose solution indicating intermediate concentration of sucrose medium as appropriate for growth of pollen tube. The addition of boric acid to sucrose medium evinced clear impact on growth of pollen tube with maximum average pollen tube length of 120.74 μ recorded in 15% sucrose medium fortified with 0.005% boric acid.
Morinda tomentosa, pollen germination, pollen viability, pollen morphology, wild noni, pollen tube
