Medicinal Plants - International Journal of Phytomedicines and Related Industries

SCOPUS
  • Year: 2017
  • Volume: 9
  • Issue: 1

The in vitro chromosomal aberrations and SCEs assays for antigenotoxic effect evaluation of the Nigella sativa L. seeds extract on cultured human peripheral blood lymphocytes

1Health and Food-Processing Industry Laboratory, Faculty of Sciences and Technics, Hassan 1st University. Casablanca Road Km 3,5 PB 539. Settat, Morocco

3National Laboratory of Scientific Police, Casablanca, Morocco

2High Institut of Health Sciences, Hassan 1st University, Casablanca Road Km 3,5 PB 539. Settat, Morocco

Abstract

In this study Methotrexate (MTX) has been used as a positive mutagen to assess the antigenotoxic effects of the Nigella sativa L. seeds extract on human peripheral blood lymphocytes. Human lymphocytes cells cultures were treated by different concentrations of the methotrexate [from 5,5.10−8 to 5,5.10 μmol/l], either alone or mixed to 80 mg/ml concentration of the N. sativa seed extract. DNA damages (cytogenetic aberrations) and cells viability were assessed by Chromosomal Aberrations (CAs) and Sister Chromatid Exchanges (SCEs) assays, Mitotic Index (MI), Stimulation Index (SI) and Replication Index (RI). Results showed that the Nigella sativa L. seed extract decreases the genotoxic effect of the MTX and possesses significant genoprotective effect of DNA by decreasing the (CAs) and increasing the (RI).

Keywords

Nigella sativa L, genotoxicity, cytogenetic, chromosomal aberrations assay, sister chromatid exchanges assay, mitotic index, stimulation index, replication index