1Horticultural Biotechnology Laboratory, KNK-College of Horticulture, Mandsaur-458001
2Department of Floriculture and Landscaping, KNK-College of Horticulture, Mandsaur-458001
3Department of Plant Pathology, KNK-College of Horticulture, Mandsaur-458001
4RVS Agricultural University, Gwalior, Madhya Pradesh, India
*Present Address: Department of Plant Molecular Biology & Biotechnology, College of Agriculture, RVS Agricultural University, Gwalior, Madhya Pradesh, India, Corresponding author e-mail: drmanojtripathi64@gmail.com
Online published on 19 July, 2017.
Three explants viz. bulb scale, leaf disc and mature embryos were cultured on different modifications of MS medium to achieve the best in vitro response. For bulb scale culture, nutrient medium MS2N.5B proved better for callus initiation. Culture medium MS.4Td exhibited higher shoot proliferating efficiency, while number of shoot (s) per explants and shoot of higher length were documented on media MS2N.5B/MS3N.5B. For cultured leaf disc, inoculation medium MS2D.5B/MS3D.5B promoted higher degree of callus induction. Higher morphogenic calli formation was exhibited by culture medium MS2N.5B/MSN.5B. However, plantlets in higher number was recovered from regeneration medium MS.5Td/MSB. For mature embryo culture, culture medium MS2D.5B proved well for callus initiation. Inoculation medium MSN.5B exhibited higher number of shoot proliferating explants. Number of shoots per explant in higher frequencies were obtained on medium MSN.5B/MSB. However, shoot of higher length was recorded on medium MSB. Higher in vitro rooting response (root proliferating efficiency, number of roots and mean root length) was exhibited by culture medium MS2IB. The plantlets were transferred to pots and hardened in Environmental Growth Cabinet and Net House during preliminary weaning period and transferred to field successfully. Phenotypic normal plants were recovered.
Amaryllis belladonna, bulb scale, mature embryo, leaf disc, organogenesis, embryogenesis and plantlet regeneration
