NEW BOTANIST - International Journal of Plant Science Research

  • Year: 2008
  • Volume: 35
  • Issue: 1–4

Gene silencing constructs for alteration of seed fatty acid composition by RNAi induced transgene silencing

  • Author:
  • Subodh Kumar Sinha, Nirupama Dubey, Archana Sachdev
  • Total Page Count: 10
  • DOI:
  • Page Number: 23 to 32

Division of Biochemistry, Indian Agricultural Research Institute, New Delhi-110012.

Abstract

With the impression to silence the endogenous fad 2–1 gene of Glycine max L. two gene silencing (Cosuppression, CS and artisense, AS) constructs were designed and produced using vicilin seed specific promoter. In co suppression and antisense, 270 bp region of fad 2–1 was taken which is conserved in FAD2 of Aarabidopsis thaliana which encodes (0–6 desaturate enzyme. Efficacy of these constructs was tested in Arabidopsis thaliana. Confirmation of integration of transgene into host genome was confirmed by PCR using bar gene. Specific primer and further by southern hybridization.

Keywords

Gene Silencing, transgene seed fatty acid