Isolation and charactreization of microsomal-6 desaturase gene from Glycine max L. cv. Pusa-9702

Using gene specific primers, a genomic sequence encoding -6 desaturase gene (fad2-1) of soybean was PCR amplified, cloned and analyzed for sequence homology. This partial genomic sequence is 191 bp long and untranslated region but has no introns and 5′ untranslated region. This partial gene fragment can be used for screening soybean genomic cDNA libraries for isolation of complete gene.