An adopter-PCR strategy for isolation and characterization of promoter from pigeonpea (Cajanus cajan L. Millsp.)

The pigeonpea cv. P33 was chosen for isolation of promoter through adopter PCR technique. A promoter (PM4) of approx. 1 kb was isolated and characterized. Sequencing and sequence analysis in silico showed presence of seed specific regulatory motifs. The promoter lacked TAT A box. Transient expression assay with GUS gene using biolistic system of gene delivery in immature seeds of mungbean and cowpea showed the ability of PM4 promoter to drive the expression of the gene fused to it. The sequence is recognized with Gene bank accession number as A Y 771784.