ORYZA- An International Journal on Rice
  • Year: 2017
  • Volume: 54
  • Issue: 2

Functional analysis of a rice tapetum specific (RTS) gene promoter variant identified in rice cultivar IR64

  • Author:
  • Narattam Sikdar1,2, Anirban Chakraborty1, Jagannath Bhattacharyya1, Saikat Chakraborty1,2, Bikas Ranjan Pati2, Soumitra Kumar Sen1, Sudip Kumar Ghosh1,3
  • Total Page Count: 10
  • Page Number: 125 to 134

1Advanced Laboratory for Plant Genetic Engineering, Indian Institute of Technology Kharagpur, Kharagpur-721302, India

2Department of Microbiology, Vidyasagar Unniversity, Medinapore(W)-721102, West Bengal, India

3Department of biotechnology, Indian Institute of Technology Kharagpur, Kharagpur-721302, West Bengal, India

Online published on 8 March, 2018.

Abstract

A novel variant of Rice Tapetum Specific (RTS) promoter, RTS64 isolated from indica rice cv. IR64, differed from reported RTS promoter from IR54 (RTS54) in the nucleotide sequence as well as in number and distribution of cis-acting regulatory elements. The new promoter contains motifs implicated in strong anther-specific expression. RTS64 promoter showed enhanced expression in anther compared to reported RTS54 promoter as indicated by Gus reporter assay. This novel anther-specific promoter, thus, would be a better candidate in genetic engineering strategies that require a high level of anther-specific expression.

Keywords

RTS54, RTS64, GUS, anther-specific expression, IR64, IR54