Progressive Agriculture

Thirty six aerobic varieties of rice collected from different agro-ecoioglcal zones were used for analysis of molecular diversity in the present study. All 36 aerobic rice varieties were grouped into two main clusters with RAPD and ISSR markers. In case of RAPD, which were grouped again into six sub-clusters. The clusterr were formed In a manner that each having 18 varletles. Out of the 6 sub clusters Govind and BW 391 were grouped in the same cluster. IR80919 and DGI 237 are clustered separately showlng differences from all the varieties/genotypes used In present study. this also signifies the geographical differences as both these are from different regions. Tarrori Basmati which Is an elite aromatic basmatl rice variety was grouped separately from other non-basmati variety we used in present study. In case of ISSR markers, these were further subdivided into 5 sub-clusters. Govind, BW391 and MAUB 13, IR71700-247-1-1-2 is grouped in different clusters respectively as in the results obtained by RAPD analysis. Same patterns follows in the case of Vallabh Basmati-21 which is clustered alone from all the varieties same as in the case of RAPD analysis. In case ISSR analysis Raipur strains are grouped in two sub-clusters rather than one as in the case of RAPD analysis. In combined analysis Vallabh Basmati 21 and Pusa Sugandha 2 are grouped In same cluster. Both the varieties are aromatic rice varieties and having similar features. These varieti"s are grouped in different clusters by individual analysis so analysis done by combine analysis found to be more reliable In clustering analysis. Finally we can conclude that no individual marker Is appropriate for genetic characterization of varieties so we should use combination of two or more markers for genetic characterization.