Progressive Agriculture

A good regeneration system is pre-requisite for any gene transfer study and likewise, an explant is a limiting factor in any plant regeneration system. In the present study, parameters like different types of leaf explants, their orientation on respective medium and portion responsible for highest regeneration frequency were studied using leaf as an explant. Explants were excised from in vitro grown axillary shoot cultures of apple rootstock MM106 and cultures on MS medium under different parametric observations. Pricked leaves with cut margin, abaxial orientation and middle portion of the leaf were evaluated with higher morphogenic capacity. Therefore, facts studied under this report like different forms of leaf explant, their orientation on the respective medium and segment responsible for highest regeneration are very much significant for carrying out any crop improvement programme through desirable gene transfer using leaf as an explant.