Effect of ABA and GA₃ on Hyperhydricity and Assessment of In Vitro and Ex Vitro Rooting Pattern for Suitability of Acclimatization in Carnation

Effect of ABA and GA₃ on hyperhydricity and shoot prolifeartion on three varieties of carnation Viz. White Sim, Exquisite and Scania on MS medium was studied. The culture system consisted of a full strength Murashige and Skoog medium with an optimum concentration of NAA (0.15 ppm) and Kinetin (1 ppm) for all three varieties. Different concentrations of ABA and GA₃ were tested for their effect on hyperhydricity and proliferation on each variety for 21 days. NAA (0.4 ppm) with GA₃ (2.0 ppm) and NAA (0.5 ppm) plus GA₃ (2.5 ppm) gave 0% hyperhydricity in all the three varieties but also reduced the shoot proliferation. All the non-hyperhydrified shoots tested for in vitro and ex vitro rooting revealed that ex vitro rooting is better.