1Department of cell Biology, College of Biotechnology, S.V.P. University of Agriculture & Technology, Meerut
2Department of Molecular Biology & Genetic Engineering, College of Biotechnology, S.V.P. University of Agriculture & Technology, Meerut
3Shobhit University, Meerut
Online published on 10 February, 2014.
Various parameters like pH, temperature, substrate concentration, incubation period and yeast extract concentration have been used in estimating the enzyme production by Aspergillus terreus. Incubation period was optimized on 8th day of fungal inoculation at 6.0 pH, 40°C temperature and 2% wheat bran was used as substrate for optimum production of xylanase. Total 5 mg/ml phosphate buffer and birch wood xylan were taken as substrate at a pH of 6.5 and the temperature of 55°C for xylanase activity. Thus xylanase preparation by this fungus was optimum at 40°C and this enzyme was found to be thermophilic in nature.
