Progressive Agriculture
  • Year: 2015
  • Volume: 15
  • Issue: 1

Effects of temperature, photoperiod and PH of culture media on in vitro shoot multiplication in sugarcane genotypes

  • Author:
  • Manoj Kumar Sharma1, R.S. Sengar1,, Pooran Chand2, Ramji Singh3, Pushpendra Kumar1, Shalini Gupta4, M.K. Yadav1
  • Total Page Count: 5
  • Page Number: 104 to 108

1Deptt. of Agri. Bio Technology, Sardar Vallabhbhai Patel University of Agriculture & Technology, Meerut, U. P.-250110 (India)

2Deptt. of Genetics & Plant Breeding, SVPUA & T., Meerut

3Deptt. of Plant Pathology, SVPUA & T., Meerut

4Deptt. of Molecular Biology and Genetic Engineering, SVPUA & T., Meerut

*Corresponding author, E-mail address: manojmeerut94@gmail.com

Online published on 4 March, 2015.

Abstract

In the present study the effects of various environmental conditions such as temperature, photoperiod and culture media pH on in vitro shoot multiplication in two sugarcane genotypes viz., Co-0238 and Co-0239 were assessed. According to the results obtained, it was concluded that temperature, photoperiod and media pH significantly affected the in vitro shoot multiplication in studied genotypes. Both genotypes showed highest shoot multiplication at temperature 25ºC, 16h/8h light/dark photoperiod and pH 6.0. Genotype Co-0238 showed highest shoot multiplication responses under different environmental conditions. This study suggests that it should be necessary to investigate the effects of above mentioned environmental conditions separately upon individual sugarcane genotypes prior to developing efficient in vitro plant regeneration protocols for commercial purposes.

Keywords

Sugarcane, Micropropagation, Genotype, pH, Photoperiod, Temperature