Progressive Agriculture

Assessment of diversity play a vital role in crop improvement. DNA markers are highly used for the study of genetic diversity, SSR (simple sequence repeats) or microsatellites are widely used to the study of genetic diversity. Total 16 rice accessions taken including land races, IRRI and WARDA developed lines. These accessions can be evaluated for agronomic traits, quality traits and molecular diversity. A total twenty random set of SSR markers are used which are developed by the Cornell University, Ithaca. For agronomic traits, significantly variability is observed. Ranging between maximum for spikelet per panicle and minimum for branches per panicle. For Quality traits maximum aroma were observed in Basmati 370 and Nagina 22 were no aroma. Alkali spreading value were observed all the accessions have high ASV and low GT except Nagina 22 and WAB197-B-6-3-12 were low ASV and high GT detected In Gel consistency test in all varieties were measured medium, where TOX3440-17-1-1-1-1-1 and WAB 99–84 showed hard gel consistency. Amylose percentage were ranged from TOX 3226-5-2-2-2 (10.61%) and the highest amylose percentage were showed WAB99–84 (21.96%). Molecular analysis, out of 360 allele, 312 were showed polymorphism with an average number of alleles 18.35 per locus.