In vitro potato plant improvement by different parts as explants

At present, a considerable progress in plant regeneration has been witnessed in potato using planttissue culture techniques. The development of plantlets through tissue culture involves a key step i.e.explant choice and the method to be adopt for crop improvement. Plantlet regeneration through theseadvanced technologies classified in two broad applications viz., direct organogenesis and through callusregeneration. Callus regeneration has been proved the efficient strategy in plant improvement bytransformation or involving genetic modification. Common growth characters of callus include complexrelationships between explants, media composition and environmental conditions during incubationperiod. Some of the callus growth has strong and difficult pages to shape, while others easily split intosmaller pieces, called "friable" callus. It may be colorless or different colors or sometimes withanthocyanin pigment as per the availability of light or dark conditions. The tissues or plant organs thatdo not normally develop callus due to cell injury, can also be induced through tissue culture techniques. Shoot induction as well as rooting are the key points in understanding the potential of tissue culturetechniques in plant improvement.