Progressive Agriculture
  • Year: 2008
  • Volume: 8
  • Issue: 2

Serological and Molecular Properties of Coat Protein Gene of Three Sugarcane Mosaic Virus Isolates From India

  • Author:
  • Pratibha Singh1,, S.K. Bhatnagar2
  • Total Page Count: 7
  • Page Number: 194 to 200

1Department of Biotechnology; D.D.U. Gorakhpur University, Gorakhpur-273009, India.

2Department of Cell Biology, College of Biotechnology, Sardar Vallabh Bhai Patel University of Agriculture and Technology, Meerut-250110, India

*Tel: 91-0551-2322108 pratibha3gkp@rediffmail.com; psgkp@hotmail.com

Abstract

Fifteen varieties of sugarcane from seven states of India were collected for screening of Sugarcane mosaic virus (SCMV) causing mosaic disease. Three varieties, i.e., Co 740, CoM 9006 and Co 62399 showed severe mosaic symptoms. In the present report, the molecular characterization of the coat protein gene. The nucleotide sequences of the coat protein for the three Indian isolates of SCMW were determined and compared with the coat protein sequences of SCMV isolates from other parts of the world. The isolates showed 19.3–94.9% identities of sequences from other parts of the world.

Keywords

Sugarcane, potyvirus, DAE-ELISA, gene analysis