Effect of Growth Regulator in Modulating the Adverse Effect of Salinity on Nitrogen Assimilating Enzymes in Pea (Pisum sativum Linn)

The study was conducted in green house at N.D. University of Agriculture and Technology, Kumar Ganj, Faizabad which lies between a latitude of 24.47° and 26.56°N and longitude of 81.12° and 83.98°E on an elevation of 113 meters above sea level in the Gangetic alluvium of eastern U.P. There was two salinity levels viz. control (0.3), 5 and 10 dSm-1 and two growth regulators with three levels like (a) Kinetin: 0, 25 and 50 mg L-i and (b) Gibberellic acid: 0, 50 and 100 mg L-1 Studies showed that biochemical nitrogen fixation severely affected by different salinity treatments. The number of nodules and leghaemoglobin content of nodules gradually and significantly reduced with increasing levels of salinity in growth medium. The seed priming with growth regulators successfully mitigate the adverse affect of salinity from all the characters. Kinetin 50 mgL-1 or GA3 100 mgL-1 treatment enhanced the nodulation and leghaemoglobin content in nodules but Kinetin treatment was superior than GA3. Higher salinity level gradually declined the nitrate and nitrite reductase, glutamine synthetase and peroxidase activity in leaves and roots whereas protease activity was accelerated. The peak values for all the enzymatic activity were found at 70 DAS accept protease enzyme.