1Department of Botany, Raja Balwant Singh College, Agra-282 002 (U.P.).
2Department of Biotechnology, SVBPUA & T, Meerut (U.P.).
The genetic diversity in nine genotypes of Brassica and their two wild relative genera Sinapis alba and Eruca sariva were studied by inter simple sequence repeats (ISSR) and simple sequence repeats (SSR) markers. A total of 10 SSRs and 4 ISSRs primers were screened. The amplification products of SSR marker were resolved on metaphor gel and product of ISSR markers were resolved on agrose gel. The amplification of genetic DNA of eleven genotypes using SSR and ISSR primers combination generated 314 bands. The UPGMA clustering method was employed to construct dendrogram based on Jaccard's similarity coefficient. The perusal of dendrogram shows the highest similarity index of 0.595 between B. campestrls var. yellow sarson and B. campestrlc var. torla. The lowest similarity coefficient (0.125) between B. napus and Eruca sativa shows that they have highest diversity between genome. B. juncea (yellow seeded) and B. juncea (brown seeded) have high similarity coefficient (0.425) that indicates the gene controlling the seed coat colour also contribute towards the genetic diversity.
Molecular marker, microsatellite, SSR and ISSR, oleiferous Brassica
