Progressive Agriculture

The protease inhibitor (PI) is known as the anti-nutrient factor which inhibits the digestive enzymes (proteases) of elementary track and also affects the normal development of different insect tarvae. The PI proteins of pigeonpea were isolated from defatted mature seeds flour of four varieties. viz. Pusa-855, Pusa-971, Pusa-33 and Pusa-84. The crude was subjected to 40–80% ammonium sulphate fractionation. The obtained precipitate dlalzyed extensively with same buffer. The dialyzed sample used for protein and PI activity estimation. After determination of PI activity for all samples Incubated on different temperature and pH. When ail the Pis were incubated at different temperature, Pusa-33 retained mora activity as compared to other varieties but on autoclave all varieties PI became Inactive wlthtn 10 minutes. On the pH scale, all PI showed simitar pattern, but retained more activity In alkaline pH. Alt the Inhibitors became inactive on autoclave and retained activity In alkaline pH. Therefore, on the consumption of human being be after cooking they will not affect their health and remain active In alkaline midgut Insect larvae.