Plant Disease Research
  • Year: 2008
  • Volume: 23
  • Issue: 2

Differentiation of Drechslera graminea isolates by random-amplified polymorphic DNA

  • Author:
  • Vineet K. Sharma, Neeru Gupta, Satvinder Kaur, Kuldeep Singh, C. Mohan
  • Total Page Count: 5
  • Page Number: 34 to 38

*School of Bio-Technology Punjab Agricultural University, Ludhiana-141004

Department of Plant Pathology, School of Bio-Technology Punjab Agricultural University, Ludhiana-141004

Online published on 25 November, 2011.

Abstract

Isolates of Drechslera graminea, the causal agent of the barley leaf stripe disease were collected from different regions of Punjab and Himachal Pradesh and analysed using randomly amplified polymorphic DNA (RAPD) markers. The genetic similarity among isolates was calculated using SAHN programme and the dendrogram constructed by the unweighted pair group method with arithmetical mean (UPGMA). The RAPD analyses showed intraspecific polymorphisms within the isolates. Drechslera graminea isolates were comprised of two major clusters, however, no correlation between the geographic origin of isolates and phylogenetic clusters was observed.

Keywords

Drechslera graminea, barley stripe disease, RAPD, DNA polymorphism, genetic diversity