National Symposium on “Plant Pathology in the Changing Global Scenario” held at National Bureau of Plant Genetic Resources, New Delhi on February 27–28, 2009
Common bean (Phaseolus vulgaris L.), the most important grain legume is severely affected by bean common mosaic virus (BCMV) belonging to family potyviridae. BCMV is the most common as well as destructive virus reducing yields wherever beans are grown and is known to possess high degree of pathogenic variability throughout the world including HP. A sound knowledge of virus and its strains is prerequisite for the development of resistant cultivars. Therefore, the main objective of this study was to study the biophysical properties and characterise the 3’ terminal region of BCMV. The identity of the test virus as NL-1 strain of BCMV was established through DAS-ELISA and reaction on international bean differential set. It was sap (90%), seed (75%) and aphid (70–80%) transmissible. Host range was restricted only to Phaseolus vulgaris, Vigna umbelleta and Vigna angularis. TIP ranged between 50–55°C, DEP 10-4-10-5, LIV 2–3 days (room temperature) and 6–7 days (4±1°C). Viral RNA was isolated from infected leaves by trizol reagent. RT-PCR amplification of total genomic RNA using BCMV degenerate primers generated an amplicon of ~1000bp, which was cloned in pGEMT-Easy vector and custom sequenced. Sequence results after blast analysis showed the product comprising of partial NIb (610bp) and partial coat protein region (363bp). The sequence has been submitted in NCBI gene bank vide accession no. EF 036693. Multiple alignment of the amplified region of the test strain by Clustal W program showed maximum homology with BCMV and BCMV strains (84–99%). This constitutes the first report of occurrence as well as sequence of NL-1 strain of BCMV in Himachal Pradesh and India.
