Powdery mildew caused by Erysiphe graminis D.C. f. sp. tritici E. Marchal is an important disease of wheat and triticale in cooler regions and is able to cause losses in yield, especially in northern and southern hill zones as well as north western plain zones. The increased use of ‘Veery’ lines in the crossing programme led to susceptibility of most of the present day cultivars in the cooler zones of India. Such cultivars have pm8 gene which is susceptible to most of the powdery mildew virulences in India. At present only DDK 1009, DDK 1029 and TL 2942 are only varieties resistant to powdery mildew, where as moderately resistant with average score of 5 are HS 420, HS 420, HS 277, VL 616, VL 738, VL 829, VL 892, HD 4672, NIDW 295, NI 5439 and MACS 2846. Keeping this in view, the search for resistant sources of powdery mildew was continued at hot spot multilocation from 2004–05 to 2007–08 crop seasons by taking 407 entries of advanced varietial trial in wheat and triticale at Shimla, Dhaulakuan, Pantnagar, Majhera, Ranichauri, Malan, Kaul, Wellington and Almora centers. These lines were screened in the form of disease screening nursery under artificially inoculated conditions using local isolates and the check entry ‘HD 2329’ was planted all over the borders of screening block as well as after every 20 lines of test entries. Each entry was planted in a row of 1 m length. The scoring was done on 0–9 scale where ‘0’denotes no disease at hard dough stage. The average and highest score of all the centres was taken into consideration in categorizing the entries for resistance. The entries showing average score ranging from 0–3 and highest score of 5 at multilocation over the years were considered resistant to powdery mildew and are listed as DDK 1009, DDK 1025, DDK 1029. HD 2189, HI 977, HPW 236, HW 1095, HW 5013, MACS 1967, TL 2934, TL 2942, VL 858. Some of the entries showed differential response to local isolates of test locations indicating the prevalence of different pathotypes. These lines may be used as differentials in pathotype analysis of powdery mildew pathogen. These lines are C306, DBW 14, HD 2189, HD 2891, HI 977, HI 1500, HI 1539, HI 8663, HI 8498, HPW 42, HPW 233, HPW 249, HS 365, HS 461, K 65, K 0307, K 9107, GW 1189, MACS 2846, MP 4010, NIDW 295, NIDW 295, NW (S) 2–4, PBW 343, PBW 373, RAJ 3765, SKW 196, UP 2691, UP 2645, VL 616, VL 738, VL 858.
