*Institute of Biological Control, BBA, Darmstadt, Germany
Department of Plant Pathology, Punjab Agricultural University, Ludhiana-141004
National Symposium on “Plant Pathology in the Changing Global Scenario” held at National Bureau of Plant Genetic Resources, New Delhi on February 27–28, 2009
Total nine isolates of Trichoderma were isolated and collected for their evaluation against white rot of peas caused by Sclerotinia sclerotiorum. First they were identified based on the morphological characters and on the basis of that the seven isolates (T1- T6 & T38) were of T. atroviride, one isolate T7 was of T. viride and one of unknown species. Further they were characterized on the molecular level by using RAPD-PCR. Two primers MB15 and MB-65 gave good amplification of DNA. Again on the basis of molecular behaviour the seven isolates i.e. T1-T6 and T38 fall under one group and the isolate T34 showed totally different behaviour. Before the biochemical characterization of all the isolates, all were screened under laboratory conditions against S. sclerotiorum by dual culture technique and poisoned food technique. On the basis of the antagonistic potentiality three most potential isolates were selected namely T1, T2 and T38. These selected isolates were further assayed for the production of four different enzymes namely, chitinases, cellulases, xylanases and glucanases on different media after 7 and 15 days of incubation and production of secondary metabolites by thin layer chromatography. Maximum production of chitinases was on the media containing colloidal chitin as carbon source followed by in the media containing sclerotia powder as carbon source. Glucanases production was more on the media amended with sclerotia powder as carbon source.
