*School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana-141004
Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana-141004
The present investigation was carried out to identify introgression lines of Triticum aestivum with Karnal bunt resistance transferred from Aegilops tauschii and their molecular characterization using PCR based D-genome specific SSR markers. A set of twelve introgression lines from cross WH890- Ae. tauschii acc. 3743 amphiploid/Veery#5//PBW343 were screened for Karnal bunt. The four lines (IL2516, IL2544, IL2568 and IL2596) out of twelve were subjected to microsatellite analysis to identify introgressed chromosome segments. Of the total 115 SSRs analyzed, 57 SSR primers were found to be polymorphic in the parents and 11 SSRs (19.3%) showed introgression from Ae. tauschii in these introgression lines. Overall, seven introgressed loci were detected in the Karnal bunt resistant introgression lines. Introgressions were observed on chromosomes 1D, 2D, 3D, 4D and 6D. The number of introgressed alleles varied from 3 to 7 in different introgression lines. Three SSR primers Xcfd15, Xcfd58 and Xwmc285 mapped on chromosome 1D and 4D, respectively were found to be linked with Karnal bunt resistance. The introgressed regions on 1DS and 4DS may carry KB resistance genes transferred from Ae. tauschii acc. 3743 to T. aestivum cv. PBW343.
Tilletia indica, Karnal bunt, wheat, Aegilops tauschii acc. 3743, microsatellites, introgression
