Plant Disease Research
  • Year: 2011
  • Volume: 26
  • Issue: 2

Comparative account of some defense related biochemicals in resistant and susceptible bean varieties during infection of Colletotrichum lindemuthianum and their correlation with pathogenicity

  • Author:
  • Anju Pathania1, B. A. Padder, O. P. Sharma, P. N. Sharma
  • Total Page Count: 1
  • Page Number: 180 to 180

1Mountain Agriculture, Research and Extension Centre, CSKHPKV, Sangla, Kinnaur

Molecular Plant Pathology laboratory Department of Plant Pathology, CSK HPKV, Palampur-176 062.

National Symposium on Strategic Issues in Plant Pathological Research held at Department of Plant Pathology, CSK HP Krishi Vishvavidayalaya, Palampur on November 24–25, 2011

Abstract

Biochemical basis of disease resistance in anthracnose (Colletotrichum lindemuthianum) resistant (G-2333, KRC-5) and susceptible (Jawala, Kanchan) common bean cultivars was studied by analysing phenols, peroxidases (PO) and Phenylalanine ammonia lyase (PAL) the important components of defense in plant kingdom. Healthy and inoculated leaf samples were collected at different time intervals for various biochemical analyses. Results indicated an initial increase in the level of total phenols, PAL (Phenylalanine ammonia lyase (PAL) and peroxidases followed by decrease with the passage of time in all the cultivars. Higher levels of total phenol, PAL and PO in uninoculated plants may be due to the constitutive defense mechanism in the plants or may be the activity of these chemicals will be more during early growth stages. Resistant cultivars possessed considerably higher amount of phenols, peroxidases and PAL as compared to susceptible ones. Among resistant cultivars, G2333 showed relatively higher activity of PAL and PO as compared to KRC-5. However, the activity of phenols was higher in KRC-5 after 144 hrs of inoculation compared to G2333. Peroxidase and PAL might have played an important role in enhanced level of phenols in inoculated varieties than un-inoculated ones.