Effect of different physiological parameters on growth and sporulation of Trichoderma viride

Most preferable temperature for the growth and sporulation of Trichoderma viride was observed upto 30°C (210.5mg dry wt. of mycelium). However, excellent sporulation was found at 25°C. The most favourable pH range was 6.5 to 7.5 having total dry weight of the mycelium between 141.5 and 160 mg, where as PDA showed excellent average colony diameter (8.12cm), and maximum mycelium weight (186.8 mg) was recorded in PDB. The in vitro antagonistic activity of Trichoderma viride against phytopathogens (Sclerotium rolfsii, Fusarium oxysporum f.s.p. ciceri, Fusarium oxysporum f.s.p. udum) was studied and it was found to be maximum against F. oxysporum f.s.p. ciceri (62.85% mycelial growth inhibition) followed by F. oxysporum f.s.p. udum (59.37% mycelial growth inhibition) and S. rolfsii (58.03% mycelial growth inhibition)