Evaluation of antibacterial potential of extracts from seven strains of Lentinus edodes

The present study was carried out to evaluate the antibacterial activity of four types of extracts (crude filtrate, alcoholic, aqueous and crude lentinan extracts) of seven strains (LeC, LeS, LeI, OE-38, OE-142, OE-329 and OE-388) of Lentinus edodes mycelium and three extracts (alcoholic, aqueous and crude lentinan) of fruiting bodies of LeS, OE-38, OE-142, OE-329 and OE-388 strains on Acinetobacter sp., E. coli, Klebsiella pneumonia, Salmonella typhii and Vibrio cholerae. The crude lentinan extracts exhibited highest antibacterial effects followed by methanolic, crude and aqueous extracts. The maximum antibacterial activity as measured by least increase in optical density was observed with crude lentinan extract of biomass of les (0.008) against Acinetobacter sp. while minimum activity was observed with aqueous extract of fruit body of OE-388 (1.251) against K. pneumoniae. All the extracts showed different antibacterial activity with different bacterial cultures. antibacterial activities of the optimized extracts from spectrophotometric methods were subsequently screened by well diffusion method. in this case, crude lentinan extracts showed maximum inhibition zones, followed by methanolic, crude and aqueous extracts.